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human cardiac microvascular endothelial cells (hmvec-c)  (Lonza)


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    Lonza human cardiac microvascular endothelial cells (hmvec-c)
    CHOP downregulation in human cardiac <t>microvascular</t> <t>endothelial</t> cells via CHOP-specific shRNAplasmid. (A) Western blot analysis of phosphorylated endothelial nitric oxide synthase (p-eNOS) was performed on cultured human cardiac microvascular endothelial cells transfected with CHOP-specific shRNA or a scrambled plasmid for 4 h. Following transfection, cells were stimulated with angiotensin II for an additional 4 h, followed by ATP stimulation for 15 min. (B) Western blot analysis of CHOP, inflammatory markers (COX2 and NLRP3), and β-actin in endothelial cells treated with CHOP-specific shRNA or scrambled plasmid for 4 h and subsequently stimulated with angiotensin II for 4 h. These data highlight the role of CHOP in modulating endothelial nitric oxide signaling and inflammatory responses under angiotensin II stimulation. Data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA followed by Tukey’s post hoc test. Non-significant (ns): p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 for comparisons between (A) CL vs. CTL + Ang-II + ATP vs. CTL + ATP, shRNA CHOP vs. shRNA CHOP + Ang-II + ATP vs. shRNA CHOP + ATP vs. shRNA scramble vs. shRNA scramble + Ang-II + ATP vs. shRNA scramble + ATP, (B) CL vs. CTL + Ang-II, shRNA CHOP vs. shRNA CHOP + Ang-II vs. shRNA scramble vs. shRNA scramble + Ang-II ( n = 3)
    Human Cardiac Microvascular Endothelial Cells (Hmvec C), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cardiac+microvascular+endothelial+cells+(hmvec-c)/human+microvascular+endothelial+cells/pmc12165939-110-4-14
    Average 90 stars, based on 1 article reviews
    human cardiac microvascular endothelial cells (hmvec-c) - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Endothelial CHOP as a central mechanism in renovascular hypertension-induced vascular endothelial dysfunction and cardiac fibrosis"

    Article Title: Endothelial CHOP as a central mechanism in renovascular hypertension-induced vascular endothelial dysfunction and cardiac fibrosis

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-025-05741-6

    CHOP downregulation in human cardiac microvascular endothelial cells via CHOP-specific shRNAplasmid. (A) Western blot analysis of phosphorylated endothelial nitric oxide synthase (p-eNOS) was performed on cultured human cardiac microvascular endothelial cells transfected with CHOP-specific shRNA or a scrambled plasmid for 4 h. Following transfection, cells were stimulated with angiotensin II for an additional 4 h, followed by ATP stimulation for 15 min. (B) Western blot analysis of CHOP, inflammatory markers (COX2 and NLRP3), and β-actin in endothelial cells treated with CHOP-specific shRNA or scrambled plasmid for 4 h and subsequently stimulated with angiotensin II for 4 h. These data highlight the role of CHOP in modulating endothelial nitric oxide signaling and inflammatory responses under angiotensin II stimulation. Data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA followed by Tukey’s post hoc test. Non-significant (ns): p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 for comparisons between (A) CL vs. CTL + Ang-II + ATP vs. CTL + ATP, shRNA CHOP vs. shRNA CHOP + Ang-II + ATP vs. shRNA CHOP + ATP vs. shRNA scramble vs. shRNA scramble + Ang-II + ATP vs. shRNA scramble + ATP, (B) CL vs. CTL + Ang-II, shRNA CHOP vs. shRNA CHOP + Ang-II vs. shRNA scramble vs. shRNA scramble + Ang-II ( n = 3)
    Figure Legend Snippet: CHOP downregulation in human cardiac microvascular endothelial cells via CHOP-specific shRNAplasmid. (A) Western blot analysis of phosphorylated endothelial nitric oxide synthase (p-eNOS) was performed on cultured human cardiac microvascular endothelial cells transfected with CHOP-specific shRNA or a scrambled plasmid for 4 h. Following transfection, cells were stimulated with angiotensin II for an additional 4 h, followed by ATP stimulation for 15 min. (B) Western blot analysis of CHOP, inflammatory markers (COX2 and NLRP3), and β-actin in endothelial cells treated with CHOP-specific shRNA or scrambled plasmid for 4 h and subsequently stimulated with angiotensin II for 4 h. These data highlight the role of CHOP in modulating endothelial nitric oxide signaling and inflammatory responses under angiotensin II stimulation. Data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA followed by Tukey’s post hoc test. Non-significant (ns): p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 for comparisons between (A) CL vs. CTL + Ang-II + ATP vs. CTL + ATP, shRNA CHOP vs. shRNA CHOP + Ang-II + ATP vs. shRNA CHOP + ATP vs. shRNA scramble vs. shRNA scramble + Ang-II + ATP vs. shRNA scramble + ATP, (B) CL vs. CTL + Ang-II, shRNA CHOP vs. shRNA CHOP + Ang-II vs. shRNA scramble vs. shRNA scramble + Ang-II ( n = 3)

    Techniques Used: Western Blot, Cell Culture, Transfection, shRNA, Plasmid Preparation

    Related Articles

    other:

    Article Title: Reengineering Ponatinib to Minimize Cardiovascular Toxicity
    Article Snippet: The human cardiac microvascular endothelial cells (HMVEC-C) were purchased from Lonza (Lonza #CC-7030, batch 0000550176), primary cell prevenient from healthy male donor, age 55 years old.

    Article Title: Biphasic influence of pravastatin on human cardiac microvascular endothelial cell functions under pathological and physiological conditions.
    Article Snippet: HMG-CoA reductase inhibitor statins are used to treat patients with hypercholesterolemia.. The pleiotropic effects of statins have been recently extended to the regulation of angiogenesis.. However, the observations on the effects of statins on endothelial cells seem to be contradictory.

    Control:

    Article Title: Shear stress activates ADAM10 sheddase to regulate Notch1 via the Piezo1 force sensor in endothelial cells
    Article Snippet: .. Human cardiac microvascular endothelial cells (HMVEC-C, CC-7030, Lonza), DAPT (D5942, Sigma-Aldrich), GI254023X (3995, Tocris Bioscience, Bristol, UK), Yoda1 (5586/10, Tocris Bioscience), ON-TARGET plus Control siRNA (Dharmacon, Lafayette, CO), siRNA Piezo1 (Sigma-Aldrich: 5’- GCAAGUUCGUGCGCGGAUU[dT][dT]- 3’), ON-TARGET plus SMARTpool human siRNA ADAM10 (Dharmacon), cleaved Notch1 Val1744 D3B8 rabbit monoclonal (4147, Cell Signaling Technology, Danvers, MA), rabbit anti-ADAM10 (AB19026, Merck KGaA, Darmstadt, Germany), goat anti human VEGFR2 (AF357, R and D system, Minneapolis, MN), mouse anti-human PECAM-1 (CD31) (M0823, Agilent Dako, Santa Clara, CA), GAPDH mouse anti-human (10R-G109b, Fitzgerald Industries International, Acton, MA) and anti-mouse, anti-rabbit and anti-goat HRP conjugated secondary antibodies (Jackson ImmunoResearch, Ely, UK). ..

    Cell Culture:

    Article Title: Oxygen consumption of human heart cells in monolayer culture.
    Article Snippet: Tissue engineering in cardiovascular regenerative therapy requires the development of an efficient oxygen supply system for cell cultures.. However, there are few studies which have examined human cardiomyocytes in terms of oxygen consumption and metabolism in culture.. We developed an oxygen measurement system equipped with an oxygen microelectrode sensor and estimated the oxygen consumption rates (OCRs) by using the oxygen concentration profiles in culture medium.



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    Image Search Results


    CHOP downregulation in human cardiac microvascular endothelial cells via CHOP-specific shRNAplasmid. (A) Western blot analysis of phosphorylated endothelial nitric oxide synthase (p-eNOS) was performed on cultured human cardiac microvascular endothelial cells transfected with CHOP-specific shRNA or a scrambled plasmid for 4 h. Following transfection, cells were stimulated with angiotensin II for an additional 4 h, followed by ATP stimulation for 15 min. (B) Western blot analysis of CHOP, inflammatory markers (COX2 and NLRP3), and β-actin in endothelial cells treated with CHOP-specific shRNA or scrambled plasmid for 4 h and subsequently stimulated with angiotensin II for 4 h. These data highlight the role of CHOP in modulating endothelial nitric oxide signaling and inflammatory responses under angiotensin II stimulation. Data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA followed by Tukey’s post hoc test. Non-significant (ns): p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 for comparisons between (A) CL vs. CTL + Ang-II + ATP vs. CTL + ATP, shRNA CHOP vs. shRNA CHOP + Ang-II + ATP vs. shRNA CHOP + ATP vs. shRNA scramble vs. shRNA scramble + Ang-II + ATP vs. shRNA scramble + ATP, (B) CL vs. CTL + Ang-II, shRNA CHOP vs. shRNA CHOP + Ang-II vs. shRNA scramble vs. shRNA scramble + Ang-II ( n = 3)

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Endothelial CHOP as a central mechanism in renovascular hypertension-induced vascular endothelial dysfunction and cardiac fibrosis

    doi: 10.1007/s00018-025-05741-6

    Figure Lengend Snippet: CHOP downregulation in human cardiac microvascular endothelial cells via CHOP-specific shRNAplasmid. (A) Western blot analysis of phosphorylated endothelial nitric oxide synthase (p-eNOS) was performed on cultured human cardiac microvascular endothelial cells transfected with CHOP-specific shRNA or a scrambled plasmid for 4 h. Following transfection, cells were stimulated with angiotensin II for an additional 4 h, followed by ATP stimulation for 15 min. (B) Western blot analysis of CHOP, inflammatory markers (COX2 and NLRP3), and β-actin in endothelial cells treated with CHOP-specific shRNA or scrambled plasmid for 4 h and subsequently stimulated with angiotensin II for 4 h. These data highlight the role of CHOP in modulating endothelial nitric oxide signaling and inflammatory responses under angiotensin II stimulation. Data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA followed by Tukey’s post hoc test. Non-significant (ns): p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 for comparisons between (A) CL vs. CTL + Ang-II + ATP vs. CTL + ATP, shRNA CHOP vs. shRNA CHOP + Ang-II + ATP vs. shRNA CHOP + ATP vs. shRNA scramble vs. shRNA scramble + Ang-II + ATP vs. shRNA scramble + ATP, (B) CL vs. CTL + Ang-II, shRNA CHOP vs. shRNA CHOP + Ang-II vs. shRNA scramble vs. shRNA scramble + Ang-II ( n = 3)

    Article Snippet: 0.3 × 10 6 Human Cardiac Microvascular Endothelial Cells (HMVEC-C, Cat No: CC-7030, from Lonza) were seeded in a six-well plate and grown in EGMTM-2MV BulletKitTM Medium complete media (Cat No: CC-3202, from Lonza) for 24 h at 37 °C, 5% CO 2 , and 95% air.

    Techniques: Western Blot, Cell Culture, Transfection, shRNA, Plasmid Preparation